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제목 기리 박사 브릭소개-doi:10.1016/j.fsi.2016.04.004
작성자 관리자
작성일자 2020-04-29
https://www.ibric.org/myboard/read.php?Board=hbs_treatise&id=46125&idauthorid=25532&stype=2&skin=agriff_treatise&BackLink=L2FncmlmZi90cmVhdGlzZV9pbmRleC5waHA/c3R5cGU9MiZzb3J0PSZTZWFyY2hZZWFyPSZTZWFyY2hNb249JnN0YXJ0eT0yMDIwJnN0YXJ0bT0wNCZQYWdlPTE1
 
Role of Bacillus subtilis VSG4-derived biosurfactant in mediating immune responses in Labeo rohita
Sib Sankar Giria, 1, Shib Sankar Senb, 1, Jin Woo Juna, Venkatachalam Sukumaranc,*, Se Chang Parka,*
a Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151742, South Korea
b School of Life Sciences, Jawharlal Nehru University, New Delhi, 110067, India
c Department of Biotechnology, Periyar Maniammai University, Thanjavur, 613403, Tamil Nadu, India
*Corresponding authors.
1 These authors contributed equally.
Abstract
This study aimed to isolate biosurfactant from CO2-sequestering Bacillus subtilis VSG4 and to evaluate its immunostimulatory effect in Labeo rohita fingerlings. Fish were injected intraperitoneally (i.p.) with 0.1 mL of phosphate-buffered saline (PBS) containing the water-soluble fraction of purified biosurfactant at 50 (S50), 100 (S100), 200 (S200), or 300 (S300) μg mL-1. Fish injected with PBS served as controls. Various immunological parameters, including immune-related gene ____expression____, were measured at 14, 21, and 28 days post administration (dpa). At 28 dpa, the fish were challenged with Aeromonas hydrophila and mortality was recorded up to 14 days. Among the immune parameters tested, lysozyme levels (36.32 ± 1.79 U mL-1), alternative complement pathway activity (76.26 ± 2.18 U mL-1), phagocytic activity (32.18 ± 0.67%), and serum bactericidal activity (73.2 ± 4.7%) were significantly higher (P < 0.05) in the S200 group at 21 dpa than in the controls. Respiratory burst activity (0.386 ± 0.008 OD630nm) was the highest in the S200 group at 28 dpa. Of the immune-related genes examined, pro-inflammatory cytokines (TNF-α and IL-1β) were significantly down-regulated in the S200 and S300 groups. ____Expression____ of anti-inflammatory cytokines (IL-10 and TGF-β) as well as IKB-α was higher (P < 0.05) in the S100 S300 groups at 21 dpa. The ____expression____ of NF-κB p65, IKK-β, MAPK p38, and Myd88 was down-regulated in the treated groups when compared to the controls. Fish in the S200 group exhibited the highest post-challenge relative survival rate (67.88%). Collectively, these results suggest that secondary metabolite (biosurfactant) isolated from B. subtilis VSG4 at 200 μg mL-1 can positively influence immune responses, enhance disease resistance, and stimulate immune-related gene ____expression____ in L. rohita.
Keywords : Bacillus subtilis VSG4; Biosurfactant; Innate immunity; Immune-related genes; Disease resistance
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