수생생물의학연구실 홈페이지입니다.

언제나 고객님의 소중한 목소리에 귀 기울이겠습니다.

고객센터

고객상담
FAQ

게시판  공지사항

고객상담


제목 기리 박사 브릭소개-doi:10.1016/j.fsi.2016.03.024
작성자 관리자
작성일자 2020-04-29
https://www.ibric.org/myboard/read.php?Board=hbs_treatise&id=45879&idauthorid=25532&stype=2&skin=agriff_treatise&BackLink=L2FncmlmZi90cmVhdGlzZV9pbmRleC5waHA/c3R5cGU9MiZzb3J0PSZTZWFyY2hZZWFyPSZTZWFyY2hNb249JnN0YXJ0eT0yMDIwJnN0YXJ0bT0wNCZQYWdlPTE1
 
 
Immunotoxicological effects of cadmium on Labeo rohita, with emphasis on the ____expression____ of HSP genes
Sib Sankar Giria, c, Shib Sankar Senb, Jin Woo Juna, Venkatachalam Sukumaranc,*, Se Chang Parka,*
a Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea
b School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
c Dept. of Biotechnology, Periyar Maniammai University, Thanjavur 613403, Tamil Nadu, India
*Corresponding authors.

Abstract
The present study evaluated the effects of exposure (28 days) to a sub-lethal concentration of cadmium (Cd) (0.65 mg CdCl2 L-1) on the immune responses and ____expression____ of immune-related and heat shock protein (HSP) genes in Labeo rohita, an important aquacultured fish species. Among the immune parameters studied, significantly lower lysozyme activity was observed in fish 28 days post-exposure (dpe) to Cd as compared to control fish. Alternative complement pathway activity was slightly higher in the Cd-exposed group at 2 dpe than in controls, and this activity declined gradually thereafter. The phagocytic activity and serum immunoglobulin M (IgM) levels were insignificantly lower in the Cd-exposed group at all assessed time points than in controls. Among serum enzymatic activities, peroxidase activity was always higher in the Cd-exposed group than in controls, but the increase was insignificant at all assessed time points. Additionally, serum glutamic-pyruvic transaminase and alkaline phosphatase activities were significantly higher in the Cd-exposed group at 14 and 28 dpe. Immune and HSP gene ____expression____ patterns were observed in kidney and liver tissues, respectively, by RT-PCR, and HSPs were further analysed by immunoblotting. Cd had an immunosuppressive effect, leading to down-regulation of TNF-α, IL-1β, IL-10, and IFN-γ. However, Cd exposure led to the up-regulation of HSP47, HSP60, HSP70, HSP78, and HSP90, indicating Cd-induced cellular stress. Taken together, the results of this study demonstrate the immunotoxic effect of Cd. Cd exposure makes Labeo rohita immunocompromised, and this could subsequently increase the disease susceptibility of Labeo rohita.
Keywords : Cadmium; Labeo rohita; Cytokine response; Toxicity; Heat shock proteins
다운로드수 0