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제목 기리 박사 브릭소개-doi:10.1016/j.fsi.2015.05.051
작성자 관리자
작성일자 2020-04-29
https://www.ibric.org/myboard/read.php?Board=hbs_treatise&id=43294&idauthorid=25532&stype=2&skin=agriff_treatise&BackLink=L2FncmlmZi90cmVhdGlzZV9pbmRleC5waHA/c3R5cGU9MiZzb3J0PSZTZWFyY2hZZWFyPSZTZWFyY2hNb249JnN0YXJ0eT0yMDIwJnN0YXJ0bT0wNCZQYWdlPTE2
 
Effect of guava leaves on the growth performance and cytokine gene ________expression________ of Labeo rohita and its susceptibility to Aeromonas hydrophila infection
Sib Sankar Giria, 1, Shib Sankar Senb, 1, Cheng Chia, Hyoun Joong Kima, Saekil Yuna, Se Chang Parka,*, V. Sukumaranc,*
a Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul-151742, South Korea
b School of Life Sciences, Jawharlal Nehru University, New Delhi-110067, India
c Dept. of Biotechnology, Periyar Maniammai University, Thanjavur-613403, Tamil Nadu, India
1 Equally contributed.
*Corresponding author : Se Chang Park, V. Sukumaranc
Abstract
The present study aimed to evaluate the effects of Psidium guajava L. (guava) leaves on the growth and immune response of the fish species Labeo rohita and its susceptibility to Aeromonas hydrophila infection. Diets containing five different concentrations of guava leaves (0% [basal diet], 0.1% [G1], 0.5% [G2], 1% [G3], and 1.5% [G4]) were fed to fish (average weight: 11.1 g) for 60 days. Various growth and immune parameters were examined 60 days post-feeding. Fish were challenged with A. hydrophila at the end of the trial, and mortalities were recorded over 15 days post-infection. We found that growth parameters such as percent weight gain (657.61 ± 9.74) and specific growth rate (3.37 ± 0.021) were significantly higher in G2 group than in the control (P < 0.05). Among the immune parameters examined, lysozyme levels (79.5 ± 5.1 U mL-1), leukocyte phagocytic activity (52% ± 4.3%), and alternative complement pathway activity (ACP) (186.1 ± 8.3 U mL-1) were significantly high (P < 0.05) in G2 fed group; there was, however, no significant effect of guava leaves at any concentration on plasma IgM level. Of the cytokine-related genes examined, interleukin-1beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) were up-regulated in the head-kidney, intestine, and hepatopancreas of fish fed experimental diets, and ________expression________ was significantly higher in G2 and G3 than in the control group. In contrast, gene ________expression________ of IL-10, transforming growth factor-beta (TGF-β), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and transcription factor nuclear factor-κB (NF-κB) were down-regulated in the treatment groups. Moreover, fish fed the G2 diet exhibited a significantly higher post-challenge survival rate (66.66%). Collectively, these results suggest that dietary supplementation with guava leaves (at 0.5% concentration) could promote growth performance and strengthen immunity of L. rohita. Guava leaves therefore represent a promising feed additive for carps in aquaculture.
Keywords : Psidium guajava; Labeo rohita; growth performance; cytokine-related genes; Aeromonas hydrophila
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