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제목 김지형 연구원 브릭소개-doi: 10.1111/tbed.12088
작성자 관리자
작성일자 2020-04-29
https://www.ibric.org/myboard/read.php?Board=hbs_treatise&id=44759&idauthorid=25959&stype=2&skin=agriff_treatise&BackLink=L2FncmlmZi90cmVhdGlzZV9pbmRleC5waHA/c3R5cGU9MiZzb3J0PSZTZWFyY2hZZWFyPSZTZWFyY2hNb249JnN0YXJ0eT0yMDIwJnN0YXJ0bT0wNCZQYWdlPTE2
 
Biological Control of Aeromonas salmonicida subsp. salmonicida Infection in Rainbow Trout (Oncorhynchus mykiss) Using Aeromonas Phage PAS-1
J. H. Kim1,2, C. H. Choresca1, S. P. Shin1, J. E. Han1, J. W. Jun1 and S. C. Park1,*
1 Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea
2 Korea Institute of Ocean Science & Technology, Ansan, Korea
* Correspondence : S. C. Park. College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
Summary
The potential control efficacy of Aeromonas phage PAS-1 was evaluated against Aeromonas salmonicida subsp. salmonicida infection in rainbow trout (Oncorhynchus mykiss) model in this study. The phage was co-cultured with the virulent A. salmonicida subsp. salmonicida strain AS05 that possesses the type III secretion system (TTSS) ascV gene, and efficient bacteriolytic activity was observed against the bacteria. The administration of PAS-1 in rainbow trout demonstrated that the phage was cleared from the fish within 200 h post-administration, and a temporal neutralizing activity against the phage was detected in the sera of phage-administrated fish. The administration of PAS-1 (multiplicity of infection: 10 000) in A. salmonicida subsp. salmonicida infected rainbow trout model showed notable protective effects, with increased survival rates and mean times to death. These results demonstrated that Aeromonas phage PAS-1 could be considered as an alternative biological control agent against A. salmonicida subsp. salmonicida infections in rainbow trout culture.
Keywords : Aeromonas salmonicida subsp. salmonicida; Aeromonas phage PAS-1; rainbow trout (Oncorhynchus mykiss); biological control

 
 
 
 
 
 
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