Plasmid profiling as a typing method for Flavobacterium psychrophilum isolates from wild and cultured freshwater fish in Japan-2008-대한수의학회 광주<?xml:namespace prefix = "o" ns = "urn:schemas-microsoft-com:office:office" />

Ji Hyung Kim, Dennis Kaw Gomez, Casiano Choresca Jr., Jee Eun Han, Sang Phil Shin, Toshihiro Nakai and Se Chang Park

대한수의학회 포스터 발표

Abstract

In order to evaluate the genetic variability of the causative agent of bacterial cold water disease, plasmid profiling was used to characterize Flavobacterium psychrophilum isolates from freshwater fish in Japan. In the first study conducted with the host freshwater fish species, the results of size analysis demonstrated that seven kinds of plasmids were present: 1.8, 2.3, 2.4, 2.7, 3.5, 23, and 100 kb. In the second study, which  involved a wild type and cultured type comparison, wild type isolates showed different plasmid profiles from the cultured type strains, and even when isolated from the same origin, F. psychrophilum showed different plasmid profiles by year. Although the function of the F. psychrophilum plasmid remained unconfirmed, these results may provide further differentiation among strains exhibiting the same ribotypes and RAPD patterns.