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연구실자료  Lab Data

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제목 Preliminary Study of Storing Fish Fin Explants-2008-대한수의학회 광주
작성자 arseidon
조회수 671

Casiano H. Choresca Jr., Ok Jae Koo, So Gun Hong , Jee Eun Han , Dennis K. Gomez, Ji Hyung Kim, Sang Phil Shin, Byeong Chun Lee and Se Chang Park

대한수의학회 poster 발표

Abstract : Somatic cells should be considered for fish genome cryobanking of valuable or endangered fish when gametes and embryos are not available. In fish, somatic cells are easily obtained from fish fin explants.  Fin explants from goldfish caudal fin were stored to various storing media, like, Leibovitz-15 (L-15), Dulbecco’s Modified Eagles Medium (DMEM), Phosphate Buffered Saline (PBS) and no storing medium. They were kept in 4 oC for 7 days, plated and cultured. After 3 days, the ability of fin explants to produce cells was assessed. Fresh fin explants (control) produced 100% donor cell explants. Fins stored in PBS (62.5%) and DMEM (55%) gave high donor cell explants and were convenient for storing. Further investigation on fin explants cryopreservation is underway in our laboratory.<?xml:namespace prefix = "o" ns = "urn:schemas-microsoft-com:office:office" />

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fin_explant_poster.ppt